Last updated: March 2026
This guide is regularly updated with the latest insights on microdosing.
What is microdosing with truffles?
Microdosing with truffles involves taking a very small amount of psilocybin-containing truffles, typically 5 to 10 percent of a regular dose. The goal is not to experience psychedelic effects, but to notice subtle improvements in creativity, focus and overall wellbeing. Research from Leiden University (2024) confirms that microdoses can stimulate both divergent and convergent thinking without cognitive side effects.
In Silicon Valley and beyond, increasing numbers of people are discovering this technique. A research team from the Netherlands, Germany and the Czech Republic studied participants at an event organised by the Psychedelic Society of the Netherlands. After a microdose of dried truffles, participants scored significantly higher on creativity measures. Their ability to generate out-of-the-box solutions increased measurably.
Effects of microdosing
With a microdose, you experience very subtle effects that come nowhere near a psychedelic trip. Hallucination is absolutely not the goal. You function normally in daily life, but may notice that certain tasks flow more easily and that you feel sharper.
The most commonly reported effects include:
- Improved focus and concentration
- Heightened alertness and mental clarity
- Enhanced creativity and problem-solving ability
- More energy without the jittery feeling of caffeine
- An elevated, positive mood
- Greater awareness of your surroundings
- Increased openness in social situations
According to a review in the Journal of Psychopharmacology (Savides & Outhoff, 2024), microdosing studies report improvements in emotional wellbeing and cognitive flexibility.
Important: Research into microdosing is still developing. If you are dealing with depression or anxiety, never replace your current treatment without consulting a doctor.
The right microdosing schedule
The most widely used protocol is the Fadiman schedule: one microdose every three days for eight to ten weeks. Your body builds tolerance to psilocybin, making daily intake pointless. The rest days are essential to maintain effectiveness.
| Day |
Fadiman Protocol |
Stamets Protocol |
Two Days Protocol |
| Monday |
Microdose |
Microdose |
Microdose |
| Tuesday |
Rest |
Microdose |
Rest |
| Wednesday |
Rest |
Microdose |
Rest |
| Thursday |
Microdose |
Microdose |
Microdose |
| Friday |
Rest |
Rest |
Rest |
| Saturday |
Rest |
Rest |
Microdose |
| Sunday |
Microdose |
Rest |
Rest |
Tip: The Fadiman protocol is the most researched and recommended for beginners. Start with this and only experiment with other schedules later.
Dosage: fresh vs. dried
The correct microdosing dosage varies per person and depends on factors like body weight, sensitivity and the potency of the truffles. It is a personal journey where you start low and gradually increase.
| Type |
Low microdose |
Standard microdose |
High microdose |
| Fresh truffles |
0.3 - 0.5 gram |
0.5 - 0.7 gram |
0.7 - 1.0 gram |
| Dried truffles |
0.1 - 0.2 gram |
0.2 - 0.3 gram |
0.3 - 0.5 gram |
Notice nothing at all? Increase slightly next time. Getting a mild 'trippy' feeling? The dose was too high. The ideal microdose sits right on the threshold: you notice subtle improvements without any visual or psychedelic effects.
Browse our microdosing truffles collection for pre-weighed portions that simplify dosing.
Preparing a microdose: step by step
Preparing a microdose requires precision. Below you will find a clear guide to preparing your truffles for microdosing.
-
Dry the truffles (optional)
If using fresh truffles, you can dry them for longer shelf life. Cut them into thin slices and place on a drying rack at room temperature for 24-48 hours, or use a food dehydrator on the lowest setting.
-
Grind the truffles into powder
Grind the dried truffles into a fine powder using a coffee grinder. This ensures an even distribution of psilocybin, enabling consistent dosing across each microdose.
-
Weigh with a precision scale
Use a scale accurate to 0.01 gram. Weigh your desired dose. For dried truffles, a typical starting dose is 0.2 gram.
-
Fill capsules (recommended)
Fill empty capsules with the weighed amount of powder. This makes consumption easier, masks the taste and ensures consistent dosing. Store capsules in an airtight container in a cool, dark place.
-
Take on an empty stomach
Take your microdose preferably in the morning on an empty stomach for optimal absorption. Wait at least 30 minutes before eating. Keep a journal to track your experiences and effects.
Ready to get started? Check out our microdosing starter pack with pre-dosed portions.
Research on microdosing (2024-2026)
Scientific research into microdosing has increased substantially in recent years. Several renowned institutions have published new findings supporting the potential of psilocybin microdoses.
Leiden University conducted a follow-up study in 2024 demonstrating that microdosing with truffles improves convergent thinking, the type of creativity needed to arrive at a single correct solution. Participants also scored higher on divergent thinking, where multiple creative solutions are generated.
Multiple studies are investigating the long-term effects of microdosing on emotional resilience. Participants in such research typically report a decrease in stress-related complaints after several weeks and an improved ability to cope with daily challenges.
Researchers at Maastricht University published results in 2025 suggesting that microdosing may promote neuroplasticity, the brain's ability to form new connections. This could explain why users report improvements in learning and memory.
Worth knowing: While results are promising, researchers emphasise that large-scale, double-blind studies are needed before definitive conclusions can be drawn. Read more about the difference between mushrooms and truffles for additional background.
Microdosing experiences
Many users who microdose with truffles report subtle but noticeable changes in their daily functioning. Commonly mentioned effects include greater mental clarity, a more stable mood and enhanced creative thinking, particularly with the Fadiman protocol (1 day on, 2 days off).
Common mistakes when microdosing
Many beginners make similar mistakes when starting with microdosing. Knowing these pitfalls helps you have a better experience.
- Dosing too high: Always start low. If you notice visual effects, the dose is too high for microdosing.
- Taking it daily: Your body builds tolerance quickly. Stick to a schedule with rest days.
- Not keeping a journal: Without notes, it is difficult to objectively assess effects and fine-tune your dose.
- Taking on a full stomach: A full stomach slows absorption. Take your microdose preferably in the morning before breakfast.
- Expecting immediate results: Most people only notice consistent changes after two to three weeks.
Also read our guide on making truffle tea, an alternative way to consume truffles that may speed up absorption.
Combinations and stacking
Some users combine microdosing with other supplements, a technique known as 'stacking'. Paul Stamets popularised combining psilocybin with lion's mane mushroom and niacin (vitamin B3).
The rationale is that lion's mane stimulates the production of nerve growth factor (NGF), whilst psilocybin promotes new neural connections. Niacin is thought to transport these compounds to the extremities of the nervous system. While science has not fully confirmed this, many users report positive results.
In our microdosing category you will find various products suitable for stacking.
Use our mushroom dosage calculator to determine your ideal microdose. Curious about the latest science? Read about psilocybin and longevity.
Why Next Level Smart?
- Microdosing truffles in ready-made strips — pre-dosed for consistent use
- Multiple truffle varieties from mild (Mexicana) to strong (Hollandia) for every experience level
- Dosage guide and protocol explanations for those new to microdosing
- Fresh truffles, vacuum-sealed with fast delivery across the Netherlands
Frequently asked questions about microdosing with truffles
How many truffles do you use for a microdose?
A typical microdose is 0.5 to 0.7 grams of fresh truffles or 0.2 to 0.3 grams of dried truffles. This is approximately 5 to 10 percent of a regular dose. Always start low and adjust based on your personal experience. Browse our
microdosing collection for pre-weighed portions.
How often can you microdose?
The most widely used schedule is the
Fadiman protocol: one microdose every three days. Day 1 you take the dose, days 2 and 3 are rest days. This prevents tolerance build-up and keeps effects consistent over a period of eight to ten weeks.
Can you combine microdosing with other supplements?
Many people combine microdosing with lion's mane mushroom and niacin, the so-called Stamets protocol. This stacking approach is intended to enhance neurological benefits. Always consult a healthcare provider if you are using other supplements or substances.
Is microdosing with truffles legal in the Netherlands?
Yes, magic truffles (sclerotia) are legally available in the Netherlands. They are not covered by the Opium Act, unlike mushrooms. You can purchase them from a smart shop or
order them online.
When will I notice the effects of microdosing?
The subtle effects of an individual microdose are typically noticeable within 30 to 60 minutes and last four to six hours. The cumulative benefits, such as improved creativity and mood, are noticed by most users after two to three weeks of consistent microdosing.
What is the difference between truffles and mushrooms for microdosing?
Truffles (sclerotia) and mushrooms contain the same active compound: psilocybin. The difference lies in their legal status and consistency. Truffles are legal in the Netherlands and have a more even psilocybin distribution, making them ideal for precise dosing. Read more in our article
mushrooms vs. magic truffles.
Can microdosing help with creativity?
Research from Leiden University shows that microdosing with truffles can improve both divergent thinking (generating multiple solutions) and convergent thinking (choosing the best solution). Many creative professionals report enhanced brainstorming sessions and increased flow states.
How do you store truffles for microdosing?
Store fresh truffles for up to two months in the fridge in their original packaging. For longer shelf life, dry and grind them into powder. Store the powder in an airtight container in a cool, dark place. This keeps them viable for months.
Last updated: March 2026 | Next Level Smart
